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Battery testing is a vital component in several industries including technology, transportation, aerospace and healthcare. At its best, it drives market-changing innovation and, in some ways, sustainability. However, incorrect battery testing can put consumer safety at risk and cost your company millions of dollars. Just look at the smartphone makers that have repeatedly recalled because of exploding batteries. This situation can be avoided with accurate, repeatable testing. This can only be achieved with a high-quality temperature and humidity test chamber. These allow you to switch between extreme conditions to measure battery durability, corrosion resistance and performance. While they range in size from 1 cubic foot to 264 cubic feet (or larger) and can be customized to your needs, the optimal temperature and humidity chambers for battery testing have the same performance and physical design. Here's what to look out for when buying. Given the number of variables that affect environmental testing, materials used to construct temperature and humidity chambers need to optimize performance and maintain durability. When considering your surroundings, you must consider extreme temperatures, humidity ranges, and potentially unstable products. The top-of-the-line Constant Temperature & Humidity Chamber has the following physical design features: High efficiency, low k-factor, thermal insulation to provide a stable temperature environment in the workspace Heavy-duty steel housing protects multiple mechanical and electrical systems from laboratory and production line elements This combination ensures accurate test results and can withstand repeated testing (as long as the chamber is calibrated regularly). You can extend the life of your test chamber beyond 15 years if you follow a regular monthly, quarterly and yearly maintenance schedule. As for performance, battery tests are typically held in a temperature range between -20 and +120 degrees Celsius. You'll find Thchamber JSB temperature and humidity chamber offers a standard range of -40 to +150 degrees Celsius. XCH JSB High And Low Temperature Humidity Chamber is through the test and detection, so as to judge whether the performance of the product meets the design requirements for testing. The criteria you test against will determine your performance needs, although it won't make any sense if the results are inaccurate. The best temperature and humidity chambers maintain accurate temperatures within 0.5 degrees Celsius and relative humidity within 2%. Fluctuations larger than those can jeopardize your data and may require further testing. Remember, finding a temperature and humidity chamber should be a collaborative effort. Consult your researchers and engineers and work with environmental chamber manufacturers with the expertise to customize solutions for you.

You may have come across terms such as environmental chambers, climate chamber, and test chamber. But what's the difference between these and which one is best for your testing needs?   In fact, these terms are interchangeable without any meaningful difference. Although an environmental chamber is probably the most commonly used term, the most important difference is determining which type of environmental testing is best for your needs. Environmental chambers need to meet a wide range of requirements, push the devices under test to their physical limits, and simulate all the real-world conditions they may encounter.   Temperature test chamber Designed to give engineers the precise control they need for temperature cycling or steady state testing, temperature chambers are used to test medical devices, pharmaceuticals, vaccines, electronics, batteries (with adjustable grips for different battery types), military and defense, communication, transportation, etc.   Constant Temperature Chamber rely on complex heating systems to ensure reliable test conditions and industry standards. Performance chambers typically have a range of -70°C to 180°C (-94°F to 356°F), while those designed for stability testing are 201°F). They come in a wide range of volumes, from benchtop models less than 1 cubic foot to walk-in rooms.   The airflow design of the chamber enables the fast-reacting heater to maintain tight control of the temperature inside the chamber to an accuracy of ±0.5°C.   The Constant Temperature Chamber also has an air-cooled or water-cooled system.   While air cooling costs less, its pull down is generally slower than water cooling options. Alternatively, water cooling units are more efficient but more complicated to install and require a permanent source of water. You can further enhance cooling by integrating liquid nitrogen (LN2) or carbon dioxide (CO2) into the system. These contribute to faster pulldown rates.   Regarding safety features, the temperature chamber has an adjustable high/low control that prevents the temperature from rising or falling below a certain limit during testing. In addition, these chambers benefit from internal high temperature safety features and a refrigeration system with overvoltage safety circuits.   All of these components combine to create an environmental test system that identifies leading-edge technology for durability and safety.   Temperature humidity test chamber In addition to temperature, many standards also require humidity components. Humidity chambers, similar to temperature chambers or thermal chambers, range in size (from bench-top to walk-in models) and performance.   They rely on steam generator systems to create and tightly control humidity conditions in the workspace.   These steam systems consist of closed stainless steel or copper pipes. Another component then heats the water that turns into steam or steam and passes through the tubes, where it combines with the conditioned air in the plenum before entering the interior workspace.   A standard humidity chamber will accommodate test conditions between 20% and 95% relative humidity (RH), which is the amount of humidity achievable at a given temperature. For example, a RH of 5 degrees Celsius is very different from an RH of 32 degrees Celsius. For those requiring more extreme RH levels, high humidity sensors can achieve up to 98% relative humidity, while desiccant air dryers can achieve as low as 5% relative humidity.   Thchamber has been designing, building and stability test chambers for many years. Get in touch today to learn more about how environmental testing can enhance your product line or help you meet industry standards.

In manufacturing, product quality is paramount. The consequences of a defect can be wide-ranging, from lost sales to costly product recalls. There is also the opportunity cost of taking the time to correct the situation. Integrating environmental testing into your product development and manufacturing process is a great way to avoid the financial and reputational consequences of premature failure. At Thchamber, we help support our customers' testing efforts to ensure safe and reliable products on the market. Below is an in-depth look at the benefits of environmental testing and how to choose the best chamber for your application. What is environmental testing? Environmental chambers are used during design or production to simulate the various climatic conditions and mechanical stresses that an item may be subjected to throughout its life cycle. Subjecting products, materials, parts, and assemblies to environmental conditions can help identify design weaknesses and set expectations. Benefits of using Environmental Test Chamber Using an environmental chamber can help companies understand how a product will perform in the real world before bringing it to market. This provides multiple benefits. Early detection of defects Improve product reliability Confirm product safety Reduce user risk Determine product life Reduce corporate liability Set the warranty period Additionally, it is used to demonstrate compliance with industry standards and government regulations. Proving that a part or assembly can withstand operating conditions goes a long way in building trust with customers. How does an environmental test chamber work? Environmental testing is performed using a variety of different types of test chambers. Specific designs vary by application, but they are usually an enclosed space equipped with the equipment needed to create unique conditions. Therefore, users can run controlled tests. These environmental test chambers range in size from small cells to spaces large enough for full vehicle testing. Common styles include: Desktop Stackable Walk-In Stability Chamber Drive-in Typical conditions for testing and their effects Generally speaking, environmental testing indicates that a product is of build quality that works flawlessly, regardless of operating conditions. Test chambers are used for different types of tests, simulating various test conditions, to meet many different types of test needs, including: Life Testing, Production Monitoring, Shock Testing, Vacuum Testing, Altitude Testing, Vibration Testing, Standards Compliance Testing, Temperature Testing, Humidity Testing, Thermal Shock Testing, Electromagnetic Radiation Testing , Low Voltage Test, Aging Test, Corrosion Test, Condensation Test, Salt Spray Test, Ingress Protection Test, Dustproof Test, Waterproof Test, RF Shielding Test Test chambers can be designed to simulate one or more environmental conditions simultaneously, increasing efficiency. If properly maintained, they can keep running for twenty years or more. How is the test chamber designed? The design and function of an environmental test chamber depends on what it will test. Some features include: glass window steel door Heating system cooling system humidification system air flow vacuum system Explosion-proof access port Shelves or shelves Choose the right test chamber When it comes to environmental inspections, choosing the right test chamber can be challenging. The device under test and the type of test you want to perform are critical to finding the best test room. Additional test room considerations include: Ability Interface Controls Budget Safety features Maintenance needs First, you should determine your testing requirements. Are there specific industry standards or government regulations that need to be met? Second, determine the size and density of the object being measured. Is a compact benchtop, walk-in or floor-standing model best for your application? When it comes to test room size, don't just think about the test room itself. The exterior dimensions need to fit the available space in your lab or facility. Next, you should weigh power requirements. The operating power of the test chamber indicates the speed at which the desired environmental conditions are reached. Leading custom test chamber manufacturer Thchamber The environmental test chambers we manufacture are used by companies in a variety of demanding industries. Examples include: car aerospace electronic product Pharmaceutical thermal technology Battery consumer product cosmetic defense medical army plastic solar sports science textile Contact us to learn more about environmental test chambers. Our experts will be happy to discuss your needs in more detail.

It would be great if you could set a testing schedule and always keep everything going according to plan - but that's not reality. If something looks wrong but the test room is still running, then maybe you can push forward and see it when you're done. Just know that all chamber systems are intertwined. Any rust or corrosion, water leaks, standing water or condensation, or air leaks are signs of a bigger, imminent repair in the future. It's important to address problems ahead of time as they arise. You can help maintain your test room by doing the following: Implement regular quarterly and annual maintenance. Run your test chamber to check performance before testing. Keep the manual and all information related to your test room nearby. Doing so will keep your test room up and running and help you catch problems before they become expensive fixes. Below are some of the more common test room maintenance checks so that you and the service team can resolve any issues that arise. Test Chamber Maintenance When you fix it early, you'll find that most chamber problems require relatively simple solutions. This is important for two reasons: You can do many maintenance tasks yourself, but it's always important to call your in-house service team, preferably someone familiar with HVAC and refrigeration. You can conduct inspections that provide context to evaluate repair options if necessary. Maintenance prevents a domino effect, where small problems turn into bigger, broader fixes. You should perform the following maintenance on a regular basis to keep your test chamber functioning properly. NOTE: Safety is paramount. The test chamber is connected to a live voltage. Any maintenance work on the electrical system should be performed by professionals. Before performing any maintenance, be sure to disconnect power to the chamber after proper lockout and tagout procedures. Electrical System: Check your electrical system for pitting and loose connections at relays, contacts, wiring harnesses, solenoids, sensor clips and compressor connections. Check that ground and phase are correct. Check all connections and terminals for electrical readings with a multimeter. View high voltage and control voltages and get necessary current readings. Test all control switches and lights for proper operation. Refrigeration system: Check oil level, look for leaks and build-up. Check various fan operations, cover ducts, duct supports and duct insulation. There should be no frost anywhere, the air handler should be sealed against leaks, and the shock absorbers should be checked. Clean the coil and straighten any bent fins. Check static and working pressures and discharges, as well as suction and liquid line temperatures. Since PSI information is specific to your chamber, measure the correct filter drier psi for limits. Test expansion tank operation, net oil pressure and high/low pressure safety checks. Humidity System: If you have a humidity chamber, check floats, water pipes and connections, and optional air dryer. Drain, clean and flush water pipes, steam generator and optional water tank. Check steam generator heater for pitting and verify water quality and pressure. Check dry air cleaning operation and filters. Circulation system: Check the pump and connecting lines for leaks. The drain tank should have only a small amount to remove sediment. Clean the pump and thoroughly clean the tank. Finally, check the overall structure of the test room: exterior, workspace and floor for punctures, tears and dents. Check all gaskets for degradation: doors, windows, ports, wiring and plugs. If you have a walk-in with panels, check the seal at each seam. Check the integrity of the panel lock or latch. Verify that door operation produces a smooth seal by inspecting latches and latches and hinges. Clean drain and condensate pumps. Clean and adjust airflow regulators, and vacuum or sweep electrical and mechanical machine areas to remove all dust and debris. Replace any missing or damaged safety labels and tags. Return the test box to the correct position, leaving 18 to 36 inches of space for optimal airflow for optimal box performance. By regularly checking your test room system, your tests will run painlessly. And the performance will always meet your expectations. Importance of Calibration In addition to the "physical" system, you should also examine the digital aspects of your room. Have an expert calibrate your chamber controller every six months or so. Regular calibration prevents "drift" that can lead to inaccurate test results. You'll avoid bad data and downtime to maintain your test plan. Remember that well-maintained Stability Test Chamber are often more durable than the controllers that enable you to operate them. The controller may require software updates and bug fixes. Don't wait to call the test room service If your test chamber is not functioning as expected, please contact your service team Stability Chamber Manufacturer Thchamber. While technicians can fix common problems in a short period of time, more complex performance issues may require in-depth solutions and may require chamber replacement. Stay ahead of the curve by performing regular maintenance, running your test box to the top and bottom of performance standards after testing or on a weekly basis (even when not in use), and staying in close contact with your service team. The more diligent you are, the more likely your Stability Chamber will remain in operation for years on end.

COVID-19 has brought dramatic changes to everyone's daily life. However, after a major hiatus of nearly two years, things seem to finally be improving. Part of the change is due to the availability of COVID-19 vaccines and booster shots to fight the pandemic. Because these vaccines are so valuable, it's important to store them properly so they can fight the virus as well as possible. In this article, you can learn about the importance of Pharmaceutical refrigerators for proper storage of COVID-19 vaccines and boosters for use in the fight against Covid-19. What type of Pharmaceutical refrigerator is used for vaccines? Because vaccines are not easy to store and are highly temperature sensitive, it is necessary to keep many of them in a Pharmaceutical refrigerator or freezer to ensure they do not spoil. Depending on the type of vaccine kept inside, their temperature range can vary widely. Typically, they run between -4F and 37F, but some specialty units may go down to -100F to safely retain certain vaccines. These refrigerators and freezers have heavy-duty doors with modern insulation and sealing gaskets to ensure consistent internal temperatures regardless of external conditions. Many will also have digital displays and warning systems to make sure you're sure the temperature is where it should be. Depending on the model, these refrigerators may also have key locks on their doors to ensure that only certain people have access to the vaccines inside. Why does a Covid vaccine need to be kept so cold? COVID-19 vaccines and boosters are produced using mRNA, which is unstable at higher temperatures. In the past, most vaccines were produced using weakened or inactive pathogens. When these vaccines are injected into the body, it boosts the immune system's response, which teaches the body to respond to bacteria or viruses. For mRNA vaccines like the COVID-19 vaccine, mRNA cells are created in the lab. These cells are designed to teach our body's cells how to make a protein to protect us from the virus we're fighting. However, the mRNA used in these vaccines is extremely fragile and breaks down rapidly. To address this issue, storing doses in medicine refrigerators has successfully extended the shelf life of vaccine doses, allowing us to extend the life of doses to months rather than hours. What are the advantages of Pharmaceutical refrigerators? The first advantage of using a Pharmaceutical refrigerator is that it keeps medicines and vaccines at the proper temperature at all times to ensure their shelf life. Medicines such as vaccines are very temperature sensitive, so keeping them at the perfect temperature is essential. Pharmaceutical refrigerators are manufactured to be very insulating and highly reliable. Another plus is that they have alarms and temperature monitors to make sure they're always at the correct temperature, and they alert the owner if they're not. Another advantage is that they are designed to promote proper air flow and not lose temperature in the event of a power outage. Finally, because they can be locked, they prevent over-opening doors and unauthorized access to sensitive medications. Thchamber has been in business for more than 15 years and continues to lead the industry in the manufacture of various pharmaceutical refrigerators and freezers as well as Environmental Chambers, Lab Vacuum Oven, Lab Biochemical Incubator, Stability Test Chamber.

Lab Biochemical Incubator for growing and maintaining microorganisms or cell cultures "The purpose of a laboratory incubator is to provide a controlled, contamination-free environment for safe and reliable work in cell and tissue culture by regulating conditions such as temperature, humidity, and carbon dioxide. Laboratory incubators are essential for the growth and storage of bacterial cultures, cell and tissue culture, biochemical and hematological research, pharmaceutical work and food analysis. " "Biochemical BOD incubators (Biological Oxygen Demand) are used to maintain temperature for testing tissue culture growth, storage of bacterial cultures and cultures that require a high degree of thermostatic accuracy. The basic difference between an incubator and a BOD incubator is temperature. Universal incubators only have a heated option and typically operate at 37°C, while BOD incubators, also known as cooled incubators, have both cooling and heating options and typically operate at low temperatures such as 10°C and 21°C. " The difference between biochemical incubator and mold incubator 1. Functional difference The biochemical incubator does not have the functions of humidity control and disinfection, while the mold incubator has both the functions of humidity control and disinfection. Therefore, a mold incubator of the same volume is slightly more expensive than a biochemical incubator. The mold incubator is equipped with a germicidal lamp, and the biochemical incubator does not need to be installed. Mold incubators are available with or without humidification, while biochemical incubators have no humidification option. Both of them can be used for bacterial culture. If the bacterial culture does not require refrigeration, an electrically heated constant temperature incubator can also be selected. 2, The difference in use Biochemical incubators are widely used in the culture and preservation of bacteria, molds, microorganisms, tissue cells, as well as water quality analysis and BOD detection, suitable for breeding experiments and plant cultivation. It is an important experimental equipment for scientific research institutions, colleges and universities, production units or department laboratories such as biology, genetic engineering, medicine, health and epidemic prevention, environmental protection, agriculture, forestry and animal husbandry. Mold incubator is an experimental equipment suitable for cultivating eukaryotic microorganisms such as mold. Since most molds are suitable for growth at room temperature (25°C), some humidity is required when growing on solid substrates. Therefore, a general mold incubator consists of a refrigeration system, a heating system, an air humidifier and a cultivation room, a control circuit and an operation panel. And use a temperature sensor and a humidity sensor to keep the temperature and humidity of the culture room stable. Some special mold incubators can also be set to change the temperature and humidity with the incubation time. Difference between Microbial incubator and Bacterial incubator Microbial incubators, also known as "heat-only" or "standard" incubators, have heating elements that provide incubation temperatures just above ambient. If the ambient temperature in the lab is around 22°C, they can only handle incubation temperatures higher than around 27°C or even 30°C. Bacterial incubator is used for the storage of bacterial plates and the growth of bacterial cultures at 37 degrees Celsius. These incubators are only equipped with heated temperatures; therefore, these are also called heated incubators. Besides, Cooling Incubator Cooling Incubator, also known as "cooled" incubators, have both cooling and heating capabilities to provide a wider temperature range - also near or even below ambient. They also typically cover a range of incubation temperatures above ambient temperature - as "microbe" or "heat-only" incubators do. The investment in refrigerated incubators is higher due to the use of more sophisticated technology.

For decades, Environmental test chamber have been used to study products with countless applications in aerospace, artificial intelligence, automotive, electronics, solar cells, medical, industrial, and consumer research. In these industries, samples or materials to be tested must be exposed to defined changes in environmental factors in order to study their effects and even prepare for future research. Conditions that an environmental test chamber can replicate are: temperature set point (or change), relative humidity or moisture in the form of rain, electromagnetic radiation, vibration, weathering, salt spray, sunlight/UV degradation, and vacuum. The type of testing involved will determine the chamber type; chambers come in a variety of sizes and are designed with different features and options. Environmental test chamber and specimen incubators Prepared samples or materials are placed in the chamber and then subjected to prescribed levels of environmental stimuli to determine their degree of response. The by-products produced are also measured and studied. Environmental test chamber can be used to test products in the following industries: automotive, building materials, chemicals, electronics, wood, cosmetics, plastics, aerospace, metals, pharmaceuticals, tobacco, textiles, packaging industry, biotechnology, biological tissue engineering, ceramics, human and veterinary medicine, food and beverages, microbiology, surface technology, and plant and insect growth. For pharmaceuticals, food and cosmetics, environmental and stability test chamber monitoring is necessary to comply with the rules set by international regulatory bodies. Environmental chambers can measure and control humidity, temperature (e.g. average dynamic temperature), differential pressure, particle counts, lighting and gas levels, and more. Advanced weathering studies in the test chamber help determine safe shelf life levels and use periods. In biology and microbiology, environmental test chambers can be used to monitor the effects of light, humidity and other factors on the growth of plants, algae, viruses, insects and small animals (fruit flies, also known as fruit flies). They are capable of culturing cells, organs and tissues, as well as plant growth and insect feeding. The aerospace industry relies on environmental test chambers to produce vacuums, thermal vacuums, and thermal experiments that simulate conditions in outer space so that space system hardware can withstand extreme stress and climatic conditions. Even astronauts' portable life support systems have been tested using environmental test chamber. Hyperbaric oxygen systems, cryogenic equipment and other instruments are used to test the effects of decompression and altitude conditions. Thchamber, a professional Environmental test Chamber manufacturer. Offering a comprehensive line of Temperature Humidity Test Chamber, thermal test chamber, low temperature test chamber, climate chamber laboratory, high temp high humidity test chamber etc. High precision. Competitive Price. Factory Direct.

Stability studies are typically performed on pharmaceuticals, food and beverages, beauty and cosmetic pharmacy issues and each ingredient to assess how they are affected by external factors such as light, heat, humidity, temperature, pressure, etc. Work to determine how these factors affect the drug. The test helps determine product shelf life and storage guidelines that are critical to consumer safety. Two common stability tests are real-time and accelerated. Live testing is done by storing the drug according to recommended conditions and inspecting or monitoring the product until it fails. Products are tested at 3, 6, 9 and 12 months in the first year, twice a year in the second year, and annually thereafter until the product fails to meet specifications and safety standards. Accelerated research requires storing products in manufacturing environments where different factors such as light or heat are accelerated to determine when a product fails. By performing accelerated studies, degradation can be predicted. XCH-TPS Photostability Test Chamber is equipped with visible light and near-ultraviolet lamp tube, Medicine Stability Chamber can independently control the type of light source, and can print and record visible light illumination and near-ultraviolet radiation in real time. Visible light and near-ultraviolet can be directly set, automatically adjusted and precisely controlled. Containers, closures or other packaging can also be tested for stability. cosmetic Thchamber Labs has received many testing requests from companies and organizations seeking stability testing, as follows: Packaging labs require plastic and polymer testing of HDPE bottles for stack load stability testing and wall thickness testing University research scientists need UK labs to test pharmaceutical compounds during stability tests on standard mouse chow. Food was formulated with this compound to a final concentration of 750 mg of drug per kg of food. To verify and establish formulation stability, we need to measure the drug concentration in the beads over a 6-month period. We need to take an initial measurement (the starting point) and then at least 2 more measurements; at the 3 month point and the 6 month point. Large companies need material labs required for UV stability testing of HDPE: orange HDPE jacket where we have to check the jacket for UV stability and jacket life European Product Safety Laboratories are required to conduct new cream testing for introduction to the EU and international markets, including safety assessment, stability testing, microbial contamination testing, challenge testing, shelf life prediction and any other testing deemed necessary. Cosmetic laboratories are required to conduct cosmetic stability testing and safety assessment testing, including compatibility and challenge testing. Nutraceutical Laboratory requires stability testing of vitamin packs sold as new water formulations. The company is sending products into large chain stores and needs to be tested for stability. See more stability testing requests If you are a product manufacturer or other organization requiring stability testing, please contact the Stability Chamber Manufacturer thchamber or submit an online testing request.

The speed at which vaccines are being developed is unprecedented during the current coronavirus pandemic. Vaccines from companies such as Biontech and Pfizer are already on the market and will be available for vaccination as soon as possible. But how long is the shelf life of a new vaccine? This is exactly what major pharmaceutical companies need to test with Thchamber's constant temperature and humidity chambers today. Typically, it takes ten to twenty years to develop a vaccine. But with the ongoing coronavirus crisis, waiting this long is not a good option. With the number of infections and deaths at consistently high levels, calls for a rapid vaccine delivery are growing. Pharmaceutical companies are struggling to keep up with demand, but are also focusing on analyzing the stability of vaccines, which are developed in years and have to be adjusted based on how the virus mutates. This is where stability testing comes in, for example in the KBF or KBF LQC, a constant temperature and humidity chamber with ICH-compliant light and light control. Smart, safe, economical and reliable: this is Thchamber. One thing is for sure: Thchamber is your reliable partner during the COVID-19 pandemic. Why stress test the new crown vaccine? The goal of pharmaceutical companies is to demonstrate to the public that vaccines are still effective over extended periods of time or after being stored at different temperatures. It's too early to tell how well the various vaccines will work after changing conditions. Constant Temperature and Humidity Chamber: Stability Test of Novel Coronavirus Vaccine Test the effectiveness of the novel coronavirus vaccine using Thchamber's constant temperature and humidity chamber. For this type of test, experts attach great importance to the uniformity of the temperature distribution inside the constant temperature and humidity chamber. Thchamber drying ovens, ultra-low temperature freezers and mildew incubators can also support you during the pandemic. Drying Oven: especially suitable for the disinfection of masks According to the federal government, masks can be reused under certain preconditions. Place the mask in a drying oven at a temperature between 65 and 70 °C for 30 minutes. In this way, the virus can be inactivated. Low temperature refrigerator: can cool down to -25°C Virus samples, such as the new coronavirus, can also be stored in our low temperature freezers. Samples are stored securely in the box and are readily available for additional testing. Incubator: ideal for cell proliferation Large numbers of cells must be grown for the new coronavirus to be tested. Constant Temperature Humidity Chamber: Stability Test of Novel Coronavirus Vaccine Large pharmaceutical companies use Thchamber's constant temperature and humidity chambers around the world to test the effectiveness of the new coronavirus vaccine. For this type of test, experts attach great importance to the uniformity of the temperature distribution inside the constant temperature and humidity chamber.

In a medical laboratory, the risk of contamination of cell cultures is immeasurable—no matter how careful the work. Miscalculation of risk is certainly not unheard of, and contamination often results in cultural loss. Therefore, in this blog, we want to shed light on how to systematically detect and avoid contamination in cell lines. Every medical laboratory is under threat day in and day out Microbial contamination in cell cultures—including those purchased from third parties—is not uncommon in laboratories. In fact, the opposite is true: Many cell lines grown in the lab are infected with mycoplasma. Tiny fungal spores lurk everywhere and can be airborne. Of course, when working in a sterile environment, there is room for human error. It's easy to make mistakes. Horror scenarios in cell culture labs - various types of contamination: Microbial contamination (bacteria, mycoplasma, fungi, yeast, etc.) virus contamination Protein contamination (prion) Chemical pollution (leachables and extractables from plastics, heavy metals, etc.) Cross-contamination with other cell cultures Where does pollution come from? 1. How "clean" was primitive culture? Problems usually start with the original material. Even with the best efforts in the production of media, some materials cannot be completely sterile. Therefore, there is always a risk of mycoplasma slipping through the sterile filter. Prions can even survive steam sterilization at 121 °C. 2. Is the working laboratory really a sterile environment? One of the main causes of laboratory contamination is the human body. For example, a lot of cross-contamination can be avoided if laboratory technicians avoid working on multiple production lines at the same time on a sterile bench. One culture can quickly infect another if the fluid is not handled properly. Also, haste is the worst enemy of sterile work. The door of the CO2 incubator should not be left open for no reason, certainly not for long periods of time. Laboratory technicians should only be working on one cell line at a time, no matter how much time pressure they are under. When unpacking single-use pipettes under the bench, once the cap is unscrewed, the cap must be set aside - keyword: Good Laboratory Practice (GLP). For more information, read our blog post: "Five exciting applications for CO2 incubators". 3. Are you using the correct laboratory equipment? Of course, it's entirely possible that even the equipment used in medical labs can lead to contamination of cell cultures. Therefore, we recommend: Use plastic containers without plasticizers Choose the right mold incubator location (locations near the washbasin can lead to soap contamination) Use incubator accessories made of biocide copper When antibiotics are used, antibiotic-free strains should be cultivated from time to time. (This is because antibiotics mask the contamination, and the infection can spread.) What investigative methods can be used to track which infections? The most dangerous thing about mycoplasma infections is that they often go undetected for long periods of time. In principle, contamination events can be controlled and tracked in a variety of ways, some of which are very complex and others less so. Experienced laboratory technicians can tell if cross-contamination has occurred simply by looking through a microscope. If we extract all the DNA from the cell culture, its mycoplasma DNA content can be detected using PCR methods. Laboratories performing viral transduction or bioassays should also check for viral contamination. Laboratories manufacturing drugs for novel treatments should check for bacteria, spores, fungi, mycoplasma, HIV, HCV, and BSE low risk. How should pollution be handled? Every instance of contamination must be recorded and graded. If nothing else, medical labs that hide their contamination problems under the rug are putting their good reputations at risk. Of course, in the event of contamination, special cleaning measures must also be taken: in the case of fungal infections, it is advisable to check whether the laboratory is regularly disinfected with alcohol-based reagents As a general rule, regular spray-disinfection or wipe-disinfection of the interior of the incubator with alcohol-based reagents will help avoid contamination Monthly hot air disinfection is standard medical practice in many laboratories In the case of sensitive stem cells, infected cell lines can only be treated with antibiotics in rare cases. In most cases, the solution is an expensive one - the culture has to be scrapped and the work has to start from scratch. In conclusion: Being able to consistently detect, verify and deal with contamination is an absolute must, especially in medical laboratories that use highly sensitive stem cells and do not use antibiotics. Transparent monitoring is critical. Covering up contamination or letting it spread only increases the danger and is unnecessary. The incubator should always be the safest component throughout the process steps; if a sample is contaminated, in most cases this occurs upstream or downstream of the incubator culture. According to the different range of temperature control, laboratory incubators are applied to different fields. Thchamber have biochemical incubator, widely used for research and production departments such as environmental protection, sanitation and epidemic prevention, agriculture, livestock and aquatic products, drug testing, cell culture, etc.

Suitable location The placement of vaccine refrigerators is critical. Adequate space is required around medical refrigerators for ventilation. For most vaccine refrigerators, about 30mm of clearance is required on each side of the refrigerator. There are several models of vaccine refrigerators that are designed not to require ventilation gaps at all. It's best to place your refrigerator in an insulated room, rather than against an exterior wall that can get hot and cold with the weather. The size of the location also affects the refrigerator capacity you can install. Appropriate The capacity of vaccine/pharmacy refrigerators needs to be able to safely store vaccines for peak demand, such as pre-flu programs. Overpackaging must be avoided as this can block cold air circulation and put vaccines at risk. For this reason, it is generally advisable to exercise caution and get a slightly larger vaccine refrigerator instead of a slightly smaller one. Vaccine and pharmacy refrigerators are divided into three main capacity ranges: – Under the counter/tabletop vaccine, the capacity of the refrigerator is usually about 130 to 160 liters. – Vertical Pharmaceutical Vaccine Refrigerators typically have a capacity of 350 to 650 liters. – Custom Pharmacy/Pharmaceutical Refrigerator with a capacity of approximately 600 liters and above. External alert If you plan to connect your vaccine refrigerator to your business's alarm system, make sure the vaccine refrigerator allows you to do so. The Thchamber refrigerator is ready for wiring to an external alarm. Thchamber refrigerator must be selected for external alarm. Data logger Consider purchasing a vaccine refrigerator with a data logger. The data logger electronically records temperature readings at set intervals. The stored data can then be downloaded to a computer. Data loggers can be used to audit and establish a record pattern of vaccine refrigerators over time. They're especially handy for determining whether refrigerators over 8 degrees Celsius are doing so within the critical 15 minutes allowed. Alert notification Thchamber refrigerators are now equipped with an alarm system that can send an alert via SMS or email if the temperature inside the refrigerator falls outside the desired range. Thchamber also offers temperature monitoring solutions that can be used with existing vaccines or laboratory refrigerators.

In Stability Testing - Overview, we look at Stability Testing Requirements - Chamber Selection, Validation Requirements, IQOQPQ and IPV Considerations. In order to demonstrate the shelf life of a drug in a certain market, the manufacturer must store it for a specified period of time at the relevant temperature and humidity. This is done in a stabilization chamber, also known as a stabilization cabinet. Regulators in each market, such as the FDA in the US, HPRA in Ireland, specify the temperature and humidity conditions that should be used and the storage time of samples, such as a minimum of 6 to 12 months. During this time, the samples were tested and their potency and degradation were measured and recorded. This is called a stability test. The most common conditions are 25°C/60%RH. For new products, accelerated conditions may be 40°C/75%RH. Other conditions include 30°C/65%RH, 30°C/35%RH and 25°C/40%RH. Another condition is 5°C ±3°C for products intended to be stored in the refrigerator. For products intended to be stored in the refrigerator, test conditions are -20°C ±5°C. Intangible Cultural Heritage ICH, the International Conference on Harmonization of Technical Requirements for the Registration of Medicinal Products for Human Use, develops rules for operational stability testing. ICH Q1A states that conditions should be held constant at ±2°C and ±5%RH during stability testing. Additionally, if these conditions are not met for more than 24 hours, the exam may have to be extended, resulting in a lot of paperwork. If conditions deviate from ±2°C/±5%RH, even for a short time, this should be stated. This may be due to the door opening to "pull" the sample. Such events are usually recorded in the room log. If there is no obvious explanation, it may be necessary to seek help from a service engineer. If the chamber fails, a quick response from the supplier is required, and although he is expected to stock major spares, the auditor also likes the site to stock some. It is also recommended to have redundancy, i.e. have another chamber on site as a backup. The chamber must be fully validated and ready to use. Photostability Test Chamber To demonstrate shelf life under ambient light conditions, samples may be exposed to precise doses of UV and visible light in specially designed chambers. ICH Q1B specifies that temperature should be controlled to prevent localized hot spots. Surveillance and 21 CFR Part 11 Indoor conditions should be independently monitored. This usually requires temperature and humidity transmitters connected to the recording system. In the light stabilization room temperature, UV and visible light intensity will be recorded. The system must comply with US 21 CFR Part 11 regulations, whether it is a paper recorder or a computer system. 21 CFR Part 11 states that all relevant data collection and storage systems must be designed to prevent counterfeiting, corruption, untraceable changes, or data loss. For computer systems, formal testing (IQOQ-Installation Qualification, Operation Qualification) should be performed to demonstrate compliance. Chamber of Commerce Qualification Likewise, formal testing of the new chamber (IQOQ and PQ performance certification) is mandatory. During its lifetime, it should be regularly maintained (usually an annual preventive maintenance service with calibration checks) and preferably mapped with multiple probes per year (IPV - Instrument Performance Verification). PQ and IPV typically require at least one 24-hour monitoring run, unloaded, loaded, or both. During this run, the display conditions must remain at the set point ±2°C, ±5%RH. Test equipment for these tests must be traceable calibrated at least annually and meet the IQOQ standard of 21 CFR P11. Stability Test chamber Selection Considerations The following factors need to be considered: Reliability; is it known, used and trusted? There should be low fluctuations in temperature and humidity capacity; planning for future needs as well as current needs footprint; footprint may be limited Do you offer local service with quick response, spare parts inventory and refrigeration repair certification? The chamber shall have integrated controls including temperature limiters and error message logging It should refill automatically. Low user maintenance Almost no consumables required Sterile moisture is generated. Microorganisms must not be injected into the chamber It should be possible to lock the keyboard There should be an output when an error occurs, which can be monitored by the building management system Should be designed in accordance with GAMP; good automated manufacturing practice IQOQ, PM and IPV have traceable calibration, are 21 CFR Part 11 compliant and should be obtained from the supplier Thchamber.